Incubation:Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Anti-human CD19 antibodies
Article Snippet: A multi-array 96-well plate (Meso Scale Discovery, P/N L15XA-3) is coated overnight at 4° C. with a 1 μg/mL solution of a goat anti-human Fc capture antibody (Jackson ImmunoResearch, P/N 109-005-098) in PBS.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 μg/mL of anti-CCL2 antibody (r2F2-SG1) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Cynomolgus monkey CCL2 calibration curve samples, quality control samples and diluted cynomolgus monkey serum samples were incubated with pH5.5 acid buffer for 10 minutes at 37° C. After that, the samples were incubated onto anti-CCL2-immobilized plate for 1 hour at 30° C. before washing.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS (−) and added to MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: TABLE 71 Number Pharmaceutical Group of animals agent Dose [mg/kg] 1 3 A375-MY201aPh/ 7.5 B167-Lamlib Single administration 2 3 A375-SCF041aPh/ 7.5 B167-Lamlib Single administration The concentration of each anti-human CD137 antibody in plasma was measured by the electrochemiluminescence (ECL) method. hCD137 (Sino Biological Inc.) was diluted with PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody (F7 (Biolegend) or clone MAB679 (R&D Systems)) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Anti-CCL2 MAB679 was used as capture antibody for samples containing humanized 11K2, 1A4 or 1A5 antibodies.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Labeling:Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Anti-human CD19 antibodies
Article Snippet: A multi-array 96-well plate (Meso Scale Discovery, P/N L15XA-3) is coated overnight at 4° C. with a 1 μg/mL solution of a goat anti-human Fc capture antibody (Jackson ImmunoResearch, P/N 109-005-098) in PBS.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 μg/mL of anti-CCL2 antibody (r2F2-SG1) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Cynomolgus monkey CCL2 calibration curve samples, quality control samples and diluted cynomolgus monkey serum samples were incubated with pH5.5 acid buffer for 10 minutes at 37° C. After that, the samples were incubated onto anti-CCL2-immobilized plate for 1 hour at 30° C. before washing.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS (−) and added to MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: TABLE 71 Number Pharmaceutical Group of animals agent Dose [mg/kg] 1 3 A375-MY201aPh/ 7.5 B167-Lamlib Single administration 2 3 A375-SCF041aPh/ 7.5 B167-Lamlib Single administration The concentration of each anti-human CD137 antibody in plasma was measured by the electrochemiluminescence (ECL) method. hCD137 (Sino Biological Inc.) was diluted with PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody (F7 (Biolegend) or clone MAB679 (R&D Systems)) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Anti-CCL2 MAB679 was used as capture antibody for samples containing humanized 11K2, 1A4 or 1A5 antibodies.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Concentration Assay:Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Anti-human CD19 antibodies
Article Snippet: A multi-array 96-well plate (Meso Scale Discovery, P/N L15XA-3) is coated overnight at 4° C. with a 1 μg/mL solution of a goat anti-human Fc capture antibody (Jackson ImmunoResearch, P/N 109-005-098) in PBS.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 μg/mL of anti-CCL2 antibody (r2F2-SG1) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Cynomolgus monkey CCL2 calibration curve samples, quality control samples and diluted cynomolgus monkey serum samples were incubated with pH5.5 acid buffer for 10 minutes at 37° C. After that, the samples were incubated onto anti-CCL2-immobilized plate for 1 hour at 30° C. before washing.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS (−) and added to MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: TABLE 71 Number Pharmaceutical Group of animals agent Dose [mg/kg] 1 3 A375-MY201aPh/ 7.5 B167-Lamlib Single administration 2 3 A375-SCF041aPh/ 7.5 B167-Lamlib Single administration The concentration of each anti-human CD137 antibody in plasma was measured by the electrochemiluminescence (ECL) method. hCD137 (Sino Biological Inc.) was diluted with PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody (F7 (Biolegend) or clone MAB679 (R&D Systems)) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Anti-CCL2 MAB679 was used as capture antibody for samples containing humanized 11K2, 1A4 or 1A5 antibodies.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Clinical Proteomics:Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Anti-human CD19 antibodies
Article Snippet: A multi-array 96-well plate (Meso Scale Discovery, P/N L15XA-3) is coated overnight at 4° C. with a 1 μg/mL solution of a goat anti-human Fc capture antibody (Jackson ImmunoResearch, P/N 109-005-098) in PBS.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 μg/mL of anti-CCL2 antibody (r2F2-SG1) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Cynomolgus monkey CCL2 calibration curve samples, quality control samples and diluted cynomolgus monkey serum samples were incubated with pH5.5 acid buffer for 10 minutes at 37° C. After that, the samples were incubated onto anti-CCL2-immobilized plate for 1 hour at 30° C. before washing.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS (−) and added to MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: TABLE 71 Number Pharmaceutical Group of animals agent Dose [mg/kg] 1 3 A375-MY201aPh/ 7.5 B167-Lamlib Single administration 2 3 A375-SCF041aPh/ 7.5 B167-Lamlib Single administration The concentration of each anti-human CD137 antibody in plasma was measured by the electrochemiluminescence (ECL) method. hCD137 (Sino Biological Inc.) was diluted with PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody (F7 (Biolegend) or clone MAB679 (R&D Systems)) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Anti-CCL2 MAB679 was used as capture antibody for samples containing humanized 11K2, 1A4 or 1A5 antibodies.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Electrochemiluminescence:Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Anti-human CD19 antibodies
Article Snippet: A multi-array 96-well plate (Meso Scale Discovery, P/N L15XA-3) is coated overnight at 4° C. with a 1 μg/mL solution of a goat anti-human Fc capture antibody (Jackson ImmunoResearch, P/N 109-005-098) in PBS.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 μg/mL of anti-CCL2 antibody (r2F2-SG1) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Cynomolgus monkey CCL2 calibration curve samples, quality control samples and diluted cynomolgus monkey serum samples were incubated with pH5.5 acid buffer for 10 minutes at 37° C. After that, the samples were incubated onto anti-CCL2-immobilized plate for 1 hour at 30° C. before washing.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS (−) and added to MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: TABLE 71 Number Pharmaceutical Group of animals agent Dose [mg/kg] 1 3 A375-MY201aPh/ 7.5 B167-Lamlib Single administration 2 3 A375-SCF041aPh/ 7.5 B167-Lamlib Single administration The concentration of each anti-human CD137 antibody in plasma was measured by the electrochemiluminescence (ECL) method. hCD137 (Sino Biological Inc.) was diluted with PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody (F7 (Biolegend) or clone MAB679 (R&D Systems)) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Anti-CCL2 MAB679 was used as capture antibody for samples containing humanized 11K2, 1A4 or 1A5 antibodies.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Blocking Assay:Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Anti-human CD19 antibodies
Article Snippet: A multi-array 96-well plate (Meso Scale Discovery, P/N L15XA-3) is coated overnight at 4° C. with a 1 μg/mL solution of a goat anti-human Fc capture antibody (Jackson ImmunoResearch, P/N 109-005-098) in PBS.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 μg/mL of anti-CCL2 antibody (r2F2-SG1) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Cynomolgus monkey CCL2 calibration curve samples, quality control samples and diluted cynomolgus monkey serum samples were incubated with pH5.5 acid buffer for 10 minutes at 37° C. After that, the samples were incubated onto anti-CCL2-immobilized plate for 1 hour at 30° C. before washing.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS (−) and added to MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: TABLE 71 Number Pharmaceutical Group of animals agent Dose [mg/kg] 1 3 A375-MY201aPh/ 7.5 B167-Lamlib Single administration 2 3 A375-SCF041aPh/ 7.5 B167-Lamlib Single administration The concentration of each anti-human CD137 antibody in plasma was measured by the electrochemiluminescence (ECL) method. hCD137 (Sino Biological Inc.) was diluted with PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody (F7 (Biolegend) or clone MAB679 (R&D Systems)) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Anti-CCL2 MAB679 was used as capture antibody for samples containing humanized 11K2, 1A4 or 1A5 antibodies.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Control:Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Anti-human CD19 antibodies
Article Snippet: A multi-array 96-well plate (Meso Scale Discovery, P/N L15XA-3) is coated overnight at 4° C. with a 1 μg/mL solution of a goat anti-human Fc capture antibody (Jackson ImmunoResearch, P/N 109-005-098) in PBS.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 μg/mL of anti-CCL2 antibody (r2F2-SG1) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Cynomolgus monkey CCL2 calibration curve samples, quality control samples and diluted cynomolgus monkey serum samples were incubated with pH5.5 acid buffer for 10 minutes at 37° C. After that, the samples were incubated onto anti-CCL2-immobilized plate for 1 hour at 30° C. before washing.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: Specifically, hCD137 (Sino Biological Inc.) was diluted in PBS (−) and added to MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC). hCD137 was added to the plate and shaken at room temperature for one hour, and hCD137 was immobilized to the plate.
Article Title: Anti-CD137 antigen-binding molecule and utilization thereof
Article Snippet: TABLE 71 Number Pharmaceutical Group of animals agent Dose [mg/kg] 1 3 A375-MY201aPh/ 7.5 B167-Lamlib Single administration 2 3 A375-SCF041aPh/ 7.5 B167-Lamlib Single administration The concentration of each anti-human CD137 antibody in plasma was measured by the electrochemiluminescence (ECL) method. hCD137 (Sino Biological Inc.) was diluted with PBS(−) and added to a MULTI-ARRAY 96-well Plate (Meso Scale Diagnostics, LLC).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody (F7 (Biolegend) or clone MAB679 (R&D Systems)) was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Anti-CCL2 MAB679 was used as capture antibody for samples containing humanized 11K2, 1A4 or 1A5 antibodies.
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
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